Chemoprotective and Nephro-Protective Effects of Manihot esculenta Methanolic Root Extract in Benzene-Induced Leukemia in Mice
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Abstract
Leukemia is a disease of the blood and bone marrow which is characterized by accumulation of white blood cells in the bone marrow and intercalation into peripheral blood causing haematological and renal disorders. The purpose of this research was to evaluate the performance of Manihot Esculenta methanolic roots extract on haematological and renal alterations in leukemia mice model. Leukemia was induced in mice with 2.0 mL of benzene solution intravenously from the tail vain 48 hourly for three consecutive weeks. The mice were divided into 6 groups; group 1-normal control received commercial feed and water, group 2- benzene positive control received feed+ water + benzene, group 3- chemotherapeutic drug control received commercial feed+ water+ benzene+ doxorubicin/ cytarabine drug regimen while group 4, 5 and 6 received feed + water + benzene and treated with 5.0 mg/kg, 10 mg/kg and 15 mg/kg Manihot esculenta roots extract. Blood samples were collected to assay for haematological indices, c-reactive proteins concentration, electrolytes parameters and blood microscopy. Blood microscopy result presented blast cells in benzene group, while haematological and electrolytes parameters showed a significant p< 0.05 increase in phosphate, white blood cells, lymphocytes, uric acid, potassium, sodium and decrease in calcium, platelets and red blood cells in benzene positive control group compared to normal control group which had normal concentrations. Treatment with Manihot Esculenta extract and doxorubicin/ cyterabine chemotherapeutic drug regimen significantly p< 0.05 reversed haematological and electrolytes parameters close to normal ranges. Therefore, Manihot Esculenta roots may be used for herbal therapeutic development or prophylaxis to cushion the effect of leukemia on kidney function and haematological indices. Although this research had limitations in carrying genetic testing like BCR-ABL1 gene therefore we recommend genetic and in-silico studies to assess the druggability of bioactive compounds in Manihot esculenta to specific genes or proteins common in leukemia.
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Copyright (c) 2025 Kebe Etim Arikpo (Author); Moses Zira Zaruwa, Iwara Arikpo Iwara, Carole chibuzor Nweze, Ajiboso Olalaikan Samiu (Translator); Christiana J. Bala (Author); Charity Onyisi Otunji, Atiku Jubril Muhammed, Vivian Oloma Abara, Shelly C. Ogbu (Translator)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Moses Zira Zaruwa, Nasarawa State University, Keffi, Nigeria.
Biochemistry department
Iwara Arikpo Iwara, University of Calabar, Nigeria.
Biochemistry department
Carole chibuzor Nweze, Nasarawa State University, Keffi, Nigeria.
Biochemistry department
Ajiboso Olalaikan Samiu, Nasarawa State University, Keffi, Nigeria.
Biochemistry department
Charity Onyisi Otunji, Nasarawa State University, Keffi, Nigeria.
Biochemistry department
Atiku Jubril Muhammed, Nasarawa State University, Keffi, Nigeria.
Biochemistry department
Vivian Oloma Abara, Nasarawa State University, Keffi, Nigeria.
Biochemistry department
Shelly C. Ogbu, Nasarawa State University, Keffi, Nigeria.
Biochemistry department
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