Chlorophyll Content of Leaves and Salt Ions (Na+ and Cl-) Content in the Seeds of Zea mays L. Treated with Different Concentrations of Salt (NaCl)
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Abstract
This study which aims at investigating the chlorophyll content of leaves and Salt ions (Na+ and Cl‑) content in the seeds of Zea mays L. treated with different concentrations of salt (NaCl) as plants are often faced with salt stress. This study was carried out at the Botanical Garden of the Department of Plant Science and Biotechnology (PSB) in Rivers State University (RSU), Port Harcourt having latitude and longitude of (4.804°N and 6.980°E), annual rainfall of (200.45mm) and annual temperature range of (25oC-27oC), Soil used for the study was collected from the aforementioned study area. Maize seeds (SAMMAZ-51) were subjected to viability test and viable seeds were sown into polybags filled with 15kg of soil at the rate of 2-seeds per bag having 4 treatments (10mM-T1, 30mM-T2, 50mM-T3, Control-T4) replicated 8-times and arranged in a Completely Randomized Design (CRD). Treatments were applied at 2-days interval and plant was allowed to grow for a period of two months before taking samples to the laboratory for chlorophyll and salt ion contents using standard methods. Chlorophyll content for Month 1 and 2 revealed T4 to have highest content (1137ug/g and 1973ug/g) while T2 had least content (724ug/g) at month I and T1 had least content (1046 ug/g) at month 2. For the salt ions content, T3 recorded highest values (359mg/kg and 400mg/kg) for sodium and chloride contents respectively, with T4 recording least values (222mg/kg and 269mg/kg) respectively for chloride and sodium contents. This study reveals that salt stress has an impact in the chlorophyll content and salt ion compositions of the different parts of maize plant studied with an increase in NaCl contents.
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