Effects of Grist Particle Size Distribution on the Wort Quality of Improved Sorghum Varieties (CSR01, CSR02, And SK5912) Under a Single-Infusion Mashing Regime
Main Article Content
Abstract
This study evaluated the effects of particle size distribution and single-infusion mashing regime on the wort quality of three improved sorghum varieties (CSR01, CSR02, and SK5912). The sorghum varieties were malted and assessed for their malting and brewing characteristics. Malted sorghum varieties were milled into different particle size fractions (coarse, medium, fine, and flour) and mashed at 60°C using a single-infusion mashing regime. Sorghum grist particle size distribution was analyzed using sieve analysis, and the resulting worts were analyzed for selected physicochemical properties. CSR01, CSR02, and SK5912 exhibited germinative capacities, germinative energy, water sensitivity, malting loss and a thousand-corn weight of 99%, 99%, 1.33%, 5.30%, and 0.24 g for CSR01 and 98%, 97%, 1.66 %, 12.12% and 0.32 g for CSR02 and 97%, 86%, 1.66%, 9.88% and 0.38g for SK5912, respectively. The values obtained in this study showed significant differences (P<0.05). The proximate composition analysis showed that CSR01, CSR02 and SK5912 exhibited moisture content, protein, lipid, ash, fibre and carbohydrate content of 7.60%, 8.55%, 5.30% 2.00%, 2.46% and 74.07% for CSR01; 8.54%, 8.19%, 5.74% 1.77%, 2.06% and 73.69% for CSR02; 11.34%, 7.69%, 6.37% 2.10%, 1.82% and 70.67% for SK5912 respectively. The values showed significant differences (P<0.05) across the varieties. Analysis of the CSR01 variety of sorghum ranged from 0.63 – 0.87 mg/ml, 4.86 – 5.66, 1.00 – 1.02, 2.26 – 2.37 and 1.04 – 2.81 ml/min for lipid concentration, pH, specific gravity, specific viscosity and filtration rate, respectively. The CSR02 variety of sorghum ranged from 0.45 – 0.66 mg/ml, 4.66 – 4.98, 1.00 – 1.02, 2.80 – 2.88 and 1.15 – 2.66 ml/min for lipid concentration, pH, specific gravity, specific viscosity and filtration rate, respectively. While the SK5912 variety ranged from 0.69 – 0.97 mg/ml, 3.87 – 4.45, 1.00 – 1.02, 2.07 – 2.17 and 1.16 – 2.29 ml/min for lipid concentration, pH, specific gravity, specific viscosity and filtration rate, respectively. There was no significant difference (P>0.05) in the specific gravity of the samples across the sorghum varieties. These results demonstrate that both sorghum variety and grist particle size influence wort quality parameters and processing performance during mashing. The findings highlight the importance of optimizing particle size distribution during sorghum malt processing to improve wort quality and support the utilization of improved sorghum varieties in brewing applications.
Article Details
Copyright (c) 2026 Etuk, Sifonobong Edem, Ogbonna, Augustine Chima, Udoh, Iniobong Enefiok, Ekanem, Kingdom Emmanuel (Author)

This work is licensed under a Creative Commons Attribution 4.0 International License.
Adebo, J. A. & Kesa, H. (2023). Evaluation of nutritional and functional properties of anatomical parts of two sorghum (Sorghum bicolor) varieties. Heliyon, 9(6):17296. DOI: 10.1016/j.heliyon.2023.e17296 DOI: https://doi.org/10.1016/j.heliyon.2023.e17296
Aliyu, A. M., Abubakar, M., Bichir, B. B., Yaqoob, R. & Shehu, F. N. (2024). Proximate and antinutritional composition of some selected sorghum (Sorghum bicolor L. Moench) varieties. Nigerian Journal of Animal Production, 11(14):127-234
American Society of Brewing Chemists (ASBC). (2011). Methods of analysis (Wort pH; Wort-Specific Gravity; Lipid analysis methods). ASBC.
AOAC INTERNATIONAL. (2023). Official methods of analysis of AOAC INTERNATIONAL (22nd ed.). Oxford University Press.
Awika, J.M. & Rooney, L.W. (2004). Sorghum Phytochemicals and Their Potential Impact on Human Health. Phytochemistry, 65, 1199-1221. DOI: 10.1016/j.phytochem.2004.04.005 DOI: https://doi.org/10.1016/j.phytochem.2004.04.001
Castillo, C., García, G., Hernández, A. & Zamora, M. (2019). Proximate composition and energy value analysis of five varieties of malting barley. International Journal of Food Science and Biotechnology, 4(2): 35. DOI: https://doi.org/10.11648/j.ijfsb.20190402.11
Correia, I., Nunes, A., Barros, A. S., & Delgadillo, I. (2008) Protein profile and malt activity during sorghum germination, Journal of the Science of Food and Agriculture, 88: 2598–2605. DOI: 10.1002/jsfa.3396 DOI: https://doi.org/10.1002/jsfa.3348
Deme, G. D., Asfaw, B. T., & Gari, M. T. (2020). Evaluation of the malting potential of different barley varieties. Journal of Water Pollution & Purification Research, 6(3), 24-35.
Dicko, M. H., Gruppen, H., Zouzouho, O. C., Traore, A. S., van Berkel, W. J. H. & Voragen, A. G. J. (2006). Effects of germination on the activities of amylases and phenolic enzymes in sorghum varieties grouped according to food end-use properties. Journal of the Science of Food and Agriculture, 86: 953–963. DOI: 10.1002/jsfa.2439 DOI: https://doi.org/10.1002/jsfa.2443
Etuk, B. R. (2003). Grist size distribution and gelatinization temperature of malted sorghum. Global Journal of Engineering Research, 2(1): 1-5. DOI: https://doi.org/10.4314/gjer.v2i1.18906
European Brewery Convention (EBC). (2010). Analytica-EBC. Fachverlag Hans Carl.
Fox, G. P. & Bettenhausen, H. M. (2023). Variation in the quality of grains used in malting and brewing. Frontiers in Plant Science, 14, 1172028. DOI: 10.3389/fpls.2023.1172028 DOI: https://doi.org/10.3389/fpls.2023.1172028
Gebreyes, B. G. (2017). Determining the physicochemical compositions of recently improved and released sorghum varieties of Ethiopia. Journal of Food and Nutrition Sciences, 5(5-1):1–5.
Hernández‐Becerra, E., Contreras‐Jiménez, B., Vuelvas‐Solorzano, A., Millan‐Malo, B., Muñoz‐Torres, C., Oseguera‐Toledo, M. E. & Rodríguez‐García, M.E. (2020). Physicochemical and morphological changes in corn grains and starch during the malting for Palomero and Puma varieties. Cereal Chemistry, 97(2):404-415. DOI: 10.1002/cche.10256 DOI: https://doi.org/10.1002/cche.10256
Houghton, J. E., Behnsen, J., Duller, R. A., Nichols, T. E. & Worden, R. H. (2024). Particle size analysis: A comparison of laboratory-based techniques and their application to geoscience. Sedimentary Geology, 464: 106607. DOI: 10.1016/j.sedgeo.2024.106607 DOI: https://doi.org/10.1016/j.sedgeo.2024.106607
Htet, M. N. S., Feng, B., Wang, H., Tian, L. & Yadav, V. (2022). Comparative assessment of nutritional and functional properties of different sorghum genotypes. Frontiers in Nutrition, 9: 1048789. DOI: 10.3389/fnut.2022.1048789 DOI: https://doi.org/10.3389/fnut.2022.1048789
Ijasan, B., Goodfellow, V., Bryce, J. H., Bringhurst, T. A., Brosnan, J. M., Jack, F. R. & Agu, R. C. (2011). Quality Assessment of a sorghum variety malted commercially under tropical conditions and controlled tropical temperatures in the laboratory. Journal of the Institute of Brewing, 117:206–211. DOI: 10.1002/j.2050-0416.2011.tb00462 DOI: https://doi.org/10.1002/j.2050-0416.2011.tb00462.x
International Association for Cereal Science and Technology (ICC). (2018). ICC Standard No. 174: Determination of germinative energy of sorghum grain. ICC. https://icc.or.at/icc-standards/standards-overview/174-standard-method
Kleinwächter, M., Meyer, A. K. & Selmar, D. (2012). Malting revisited: Germination of barley (Hordeum vulgare L.) is inhibited by both oxygen deficiency and high carbon dioxide concentrations. Food Chemistry, 132(1), 476-481. DOI: 10.1016/j.foodchem.2011.10.108 DOI: https://doi.org/10.1016/j.foodchem.2011.11.027
Krstanović, V., Habschied, K., Košir, I. J. & Mastanjević, K. (2023). The Influence of Texture Type and Grain Milling Degree on the Attenuation Limit, Protein Content, and Degradation in Wheat Wort. Applied Sciences, 13(19):10626. DOI: 10.3390/app131910626 DOI: https://doi.org/10.3390/app131910626
Lakurbe, O. A., Doma, U. D., Abubakar, M., Bello, K. M., Sadiq, M. S., Yakubu, I. & Tarki, S. K. (2020). Comparative effects of sorghum SK-5912 (Sorghum bicolor, L. Moench) and pearl millet as dietary energy sources on performance and economics of production of broiler chickens. Nigerian Journal of Animal Production, 47(1):161 - 166 DOI: https://doi.org/10.51791/njap.v47i1.223
Mawouma, S., Condurache, N. N., Turturică, M., Constantin, O. E., Croitoru, C. & Rapeanu, G. (2022). Chemical composition and antioxidant profile of sorghum (Sorghum bicolor (L.) Moench) and pearl millet (Pennisetum glaucum (L.) R. Br.) grains cultivated in the Far North region of Cameroon. Foods, 11(14): 123-134. DOI: 10.3390/foods11142123 DOI: https://doi.org/10.3390/foods11142026
Mitteleuropäische Brautechnische Analysenkommission (MEBAK). (2016). Germinative energy in barley (BRF method). MEBAK – Raw material methods. https://www.mebak.org
Nocente, F., Galassi, E., Taddei, F., Natale, C., & Gazza, L. (2024). Preliminary Evaluation of Minor Cereals as Non-Traditional Brewing Raw Materials. Beverages, 10(1):2 DOI: 10.3390/beverages10010002 DOI: https://doi.org/10.3390/beverages10010002
Ogbonna, A. C. (2011). Current developments in malting and brewing trials with sorghum in Nigeria: A review. Journal of the Institute of Brewing, 117:394–400. DOI: 10.1002/j.2050-0416.2011.tb00485 DOI: https://doi.org/10.1002/j.2050-0416.2011.tb00485.x
Ogbonna, A. C., Obi, S. K. C. & Okolo, B. N. (2004). Optimization of proteolytic activities in malting sorghum. Process Biochemistry, 39(6): 711-716. DOI: 10.1016/S0032-9592(03)00180-7 DOI: https://doi.org/10.1016/S0032-9592(03)00181-X
Ogbonna, A. C., Obi, S. K. C. & Okolo, B. N. (2003) Protein modification in malting sorghum. World Journal of Microbiology and Biotechnology, 19:495–503. DOI: 10.1023/A:1025178303028 DOI: https://doi.org/10.1023/A:1025189713390
Ogu, E. O., Odibo, F. J., Agu, R. C. & palmer, G. H. (2006). Quality assessment of different sorghum varieties for their brewing potential. Journal of the Institute of Brewing, 112:117-121. DOI: 10.1002/j.2050-0416.2006.tb00238.x DOI: https://doi.org/10.1002/j.2050-0416.2006.tb00241.x
Okonkwo, I. F., Okoro, N. C. N. & Izundu, M. I. (2021). Evaluation of the Grain and Malting Properties of Four Recently Improved Sorghum Varieties. The Bioscientist Journal, 9(1): 51-63.
Orji-Udezuka, A. C., Chukwurah, E.N. & Ezemba, C.C. (2020). Evaluation of Selected Nigerian Sorghum Malt Extract Quality as an Alternative to Barley in Beer Industries. African Journal of Education, Science and Technology, 5(4):2-13
Owuama, C. I. (1997) Sorghum: A cereal with lager beer brewing potential. World Journal of Microbiology and Biotechnology, 13:253–260. DOI: 10.1023/A:1018591316654 DOI: https://doi.org/10.1023/A:1018566503879
Palmer, G. H. (1989) Cereals in malting and brewing, in Cereal Science and Technology (G. H. Palmer Ed.), Aberdeen University Press, Aberdeen, 61–242
Palombi, L., Tufariello, M., Durante, M., Fiore, A., Baiano, A. & Grieco, F. (2023). Assessment of the impact of unmalted cereals, hops, and yeast strains on volatolomic and olfactory profiles of Blanche craft beers: A chemometric approach. Food Chemistry,416: 135783. DOI: 10.1016/j.foodchem.2023.135783 DOI: https://doi.org/10.1016/j.foodchem.2023.135783
Pérez‐Carrillo, E. & Serna‐Saldívar, S. O. (2007). Effect of protease treatment before hydrolysis with α‐amylase on the rate of starch and protein hydrolysis of maize, whole sorghum, and decorticated sorghum. Cereal Chemistry, 84(6): 607-613. DOI: 10.1094/CCHEM-84-6-0607 DOI: https://doi.org/10.1094/CCHEM-84-6-0607
Taylor, J. R. N. & Duodu, K. G. (2015). Sorghum and millets: Grain-quality characteristics and management of quality requirements. In Cereal Grains (Woodhead Publishing), 317–351. DOI: 10.1016/B978-1-78242-158-0.00012-7 DOI: https://doi.org/10.1016/B978-0-08-100719-8.00013-9